if you use any port over 49999 (aka 50000 and up) for Tixati it will give this error.
Then something blocks Tixati taking that port (Windows, firewall, port already taken etc.) or something outside blocks traffic for that port (ISP or VPN, maybe it's already taken on their end?).
I've had some of these port checker tools lie to me. If Tixati says port is blocked/unconfirmed then it really is. Port-forwarding is hard to get right but it's essential to get Bittorrent to work flawlessly.